Endocrine Abstracts

Breakpoint cluster region abelson fusion oncoprotein (BCR-ABL) targeted tyrosine kinase inhibitors (TKIs) are widely used in long-term medications of hematological malignancies. Numerous clinical observations confirmed that these drugs significantly modify the physiology of bone tissue as a side effect. Currently, there are numerous contradictory results regarding the complex effects of TKIs on bone metabolism, as well as there is no clear evidence to explain them, either in t...

In hypoparathyroidism, inadequate levels of parathyroid hormone (PTH) result in hypocalcemia and often hyperphosphatemia. Large doses of calcium (Ca) and active vitamin D are typically used to manage symptoms, although these are often associated with complications and do not address the underlying PTH deficiency. In the phase III REPLACE trial, 24 weeks of treatment with rhPTH(1–84) was associated with maintained serum Ca without urine Ca excretion increases despite clini...

Background: Adequate vitamin D supply is necessary for bone metabolism and also for the general maintenance of health. 25OHD is transported primarily bound to vitamin-D binding protein (DBP), but also to albumin. Lower concentration of plasma proteins may cause difficulties in immunoanalytics. Our aim was to investigate the protein dependence of the two most often used 25OHD immuno- (CLIA) and protein binding- (ECLPBA) assays.Methods: levels were measure...

Objectives: We previously published the result of CYP24A1 gene expression in one hundred, solely papillary thyroid carcinoma (PTC) compared to its own tumour free control from the same patient. We report an increase in CYP24A1 gene transcription in more than half of analysed PTCs. Elevated CYP24A1 protein expression was also observed in the cancerous tissue section compared to peritumoural normal thyroid tissue. In the present study we aimed to examine CYP24A1 gene transcripti...

Background: Despite receiving biochemically adequate levothyroxine replacement, many patients with primary hypothyroidism suffer from persistent symptoms and a reduced quality of life (QoL). The underlying causes of this phenomenon are not fully understood, but psychological factors, autoimmune inflammation, and variations in thyroxine conversion have all been suggested as potential contributors. At this time, however, there is no consensus on the root cause.<p class="abst...

Background: The ’three-genes’ (SFN, MRC2, HMGA2) might have direct role in various cancer progression and malignant thyroid conditions. We previously reported an increased expression of 1,25-D3 neutralizing enzyme, (24-hydroxylase – CYP24A1) as a possible novel marker gene in thyroid carcinoma. In this study, we examined the transcription activity of CYP24A1 and ‘three-genes’ in four functional subgroups of papillary thyroid tumor (PTC)....

Cold nodules are one of the most common findings on scintiscans and ultrasound examinations of the thyroid gland. About 5-10% of these nodules turn out to be histologically malignant. Our aim was to examine the predictive value of somatogenetic alterations associated with thyroid cancer in FNA samples of thyroid cold nodules being citologically benign at the beginning of the study. These alterations included single nucleotide mutations (BRAF, HRAS, NRAS, KRAS) and genetic tran...

Thyroid cancer is the most common malignancy of endocrine organs and its incidence is steadily growing worldwide. Approximately 80% of differentiated thyroid tumors are papillary carcinomas. Next-generation sequencing (NGS) allows for high-throughput sequencing analysis of large number of samples at a time in a cost effective manner. We have developed a targeted sequencing parallel testing panel for multiple mutations in genes involved in thyroid cancer pathology. A custom-mad...

Cold nodules are one of the most common findings on scintigraphic examinations of the thyroid gland. About 5–10% of these nodules turn out to be histologically malignant. Our aim was to examine some somatogenetic alterations associated with thyroid cancer in FNA samples of the thyroid. These alterations included single nucleotide mutations (BRAF, HRAS, NRAS, KRAS) and genetic translocations (RET/PTC1, RET/PTC3, PAX8ex7/PPARgamma, PAX8ex9/PPARgamma). The SNPs were tested b...

Cold nodules are one of the most common findings on scintigraphic examinations of the thyroid gland. About 5–10% of these nodules turn out to be histologically malignant. Our aim was to examine some somatogenetic alterations associated with thyroid cancer in FNA samples of the thyroid. These alterations included single nucleotide mutations (BRAF, HRAS, NRAS, KRAS) and genetic translocations (RET/PTC1, RET/PTC3, PAX8ex7/PPARgamma, PAX8ex9/PPARgamma). The SNPs were tested b...